For any questions please use our Phusion Request for Technical Support or call NEB Technical Support at 1-800-632-7799. Phusion® is a registered trademark and property of Thermo Fisher Scientific. Fidelity assays were performed using a lacI-based method modified from Frey Suppman 1995.
Phusion® Technology. Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme even on your most difficult templates. Additionally Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA
Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer 5x Phusion GC Buffer DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration. Quantity. 500U (2U/µL) Concentration. 2 U/µL.
2020-7-25 · NEB Tm Calculator. Product Group. Q5 Q5 Hot Start Q5U Hot Start OneTaq OneTaq Hot Start Hot Start Taq Taq DNA Polymerase LongAmp Taq LongAmp Hot Start Taq Hemo KlenTaq Vent Deep Vent Master Mix Phusion Phusion Hot Start Flex EpiMark Hot Start. Polymerase/Kit. Q5 High-Fidelity DNA Polymerase Q5 High-Fidelity 2X Master Mix. Primer Concentration
Phusion. From Synthetic construct (aliases Phusion DNA Polymerase) Phusion is an enzyme of unknown family from Synthetic construct . test.
Phusion High-Fidelity PCR Master Mix with GC Buffer offers robust performance and can be used for all PCR applications. It is a 2X master mix consisting of Phusion DNA Polymerase deoxynucleotides and reaction buffer that has been optimized and includes MgCl 2. All that is required is the addition of template primers and water.
Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity 3´→ 5´ exonuclease activity and will generate blunt-ended products. Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer as well as 5X Phusion GC Buffer which can be used for complex or GC-rich templates.
Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1) Phusion is one of the most accurate thermostable polymerases available.
Phusion ® DNA Polymerase exploits this dramatic increase in processivity resulting in shorter extension times more robust and high yield amplification and the ability to amplify long templates in a fraction of the time. Click here to acc ess our Tm calculator. Phusion ® was developed by Finnzymes Oy now a part of Thermo Fisher Scientific
2021-6-18 · NEB()Phusion DNA Phusion DNA .cpmsbt border-bottom 1px solid #CC9966color #CC9933font-family Verdanafont-size
2017-8-18 · Proof (800bp) Tried homemade buffer #1 vs storebought NEB 5X HF buffer with homemade phusion PCR for mTAG-BFP2 from 25 ng plasmid. Overall it worked if I m being picky there seems to be a bit more smearing in the homemade buffer however the amount of desired product is the same between the two buffers.
Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity 3´→ 5´ exonuclease activity and will generate blunt-ended products. Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer as well as 5X Phusion GC Buffer which can be used for complex or GC-rich templates.
2021-7-14 · • The Phusion ™ High–Fidelity DNA Polymerase should be pipetted carefully and gently as the high glycerol content (50 ) in the storage buffer may otherwise lead to pipetting errors. • Due to the nature of the Phusion ™ High–Fidelity DNA Polymerase the optimal reaction conditions may differ from PCR protocols for standard DNA
Phusion Hot Start Flex DNA Polymerase is available as standalone enzyme or in a master mix format and enables high specificity amplification of a broad range of templates. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. NEB offers a guidelines for choosing the correct DNA polymerase for
2010-10-12 · 2x Phusion PCR Master Mix contains 0.04 U/µl Phusion High-Fidelity DNA Polymerase 2x Phusion HF Buffer (in F-531) or 2x Phusion GC Buffer (in F-532) and 400 µM of each dNTP. Thermostable Phusion DNA Polymerase is isolated and purified from an Eli strain carrying a plasmid with the cloned Phusion DNA Polymerase gene. Phusion DNA
Phusion Hot Start Flex DNA Polymerase is unlike other enzymes and care must be taken when designing cycling protocols. To determine the optimal annealing temperatures for a given set of primers use of the NEB Tm Calculator is highly recommended. Product Source An E. coli strain that carries the Phusion DNA Polymerase gene. Reagents Supplied
Phusion. GC Buffer Pack. New England Biolabs supplies a unique 5X reaction buffer and supplemental reagents for use with Thermo Scientific® Phusion High-Fidelity DNA Polymerase and associated products all of which are manufactured and quality controlled at New England Biolabs.
This page presents all the information in Polbase for Phusion Hot Start HF. Mutants At the top of the page you ll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section. Structures/Sequence If available a representative structure is
2020-7-25 · NEB Tm Calculator. Product Group. Q5 Q5 Hot Start Q5U Hot Start OneTaq OneTaq Hot Start Hot Start Taq Taq DNA Polymerase LongAmp Taq LongAmp Hot Start Taq Hemo KlenTaq Vent Deep Vent Master Mix Phusion Phusion Hot Start Flex EpiMark Hot Start. Polymerase/Kit. Q5 High-Fidelity DNA Polymerase Q5 High-Fidelity 2X Master Mix. Primer Concentration
Annealing temperatures required for use with Phusion tend to be higher than with other PCR polymerases. The NEB Tm calculator should be used to determine the annealing temperature when using Phusion. Typically primers greater than 20 nucleotides in length anneal for 10–30 seconds at 3°C above the T m of the lower T m primer.
For any questions please use our Phusion Request for Technical Support or call NEB Technical Support at 1-800-632-7799. Phusion® is a registered trademark and property of Thermo Fisher Scientific. Fidelity assays were performed using a lacI-based method modified from Frey Suppman 1995.
2021-6-18 · NEB()Phusion DNA Phusion DNA .cpmsbt border-bottom 1px solid #CC9966color #CC9933font-family Verdanafont-size
Phusion High-Fidelity PCR Master Mix with GC Buffer offers robust performance and can be used for all PCR applications. It is a 2X master mix consisting of Phusion DNA Polymerase deoxynucleotides and reaction buffer that has been optimized and includes MgCl 2. All that is required is the addition of template primers and water.
Manufactured and quality-controlled at New England Biolabs Thermo Scientific ® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. 50X higher fidelity than Taq. Robust reactionsmaximal success with minimal optimization. Offered with multiple buffers for customized reaction setup for different DNA templates.
Phusion® Technology. Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme even on your most difficult templates. Additionally Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA
Phusion ® DNA Polymerase exploits this dramatic increase in processivity resulting in shorter extension times more robust and high yield amplification and the ability to amplify long templates in a fraction of the time. Click here to acc ess our Tm calculator. Phusion ® was developed by Finnzymes Oy now a part of Thermo Fisher Scientific
Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase deoxynucleotides and reaction buffer that has been optimized and includes MgCl 2. All that is required is the addition of template primers and water. Fidelity assays were performed using a lacI-based method modified from Frey Suppman
2020-8-14 · Phusion DNA Polymerase in Phusion HF Buffer is determined to be 4.4 x 10-7 which is approximately 50-fold lower than that of Thermus aquaticus DNA polymerase and 6-fold lower than that of Pyrococcus furiosus DNA polymerase. Phusion DNA Polymerase possesses the following activities 5´→3´ DNA polymerase activity and 3´→5´ exonuclease
2017-8-18 · Proof (800bp) Tried homemade buffer #1 vs storebought NEB 5X HF buffer with homemade phusion PCR for mTAG-BFP2 from 25 ng plasmid. Overall it worked if I m being picky there seems to be a bit more smearing in the homemade buffer however the amount of desired product is the same between the two buffers.
For any questions please use our Phusion Request for Technical Support or call NEB Technical Support at 1-800-632-7799. Phusion® is a registered trademark and property of Thermo Fisher Scientific. Fidelity assays were performed using a lacI-based method modified from Frey Suppman 1995.
Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1) Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity 3´→ 5´
2017-8-18 · Proof (800bp) Tried homemade buffer #1 vs storebought NEB 5X HF buffer with homemade phusion PCR for mTAG-BFP2 from 25 ng plasmid. Overall it worked if I m being picky there seems to be a bit more smearing in the homemade buffer however the amount of desired product is the same between the two buffers.
Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error-rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1) Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity 3´→ 5´
Phusion® Technology. Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme even on your most difficult templates. Additionally Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA
Phusion® Technology. Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme even on your most difficult templates. Additionally Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA
2021-6-23 · Choose a DNA RNA genome editing qPCR calculator from NEB a leader in production and supply of reagents for the life science industry.
Phusion ® DNA Polymerase exploits this dramatic increase in processivity resulting in shorter extension times more robust and high yield amplification and the ability to amplify long templates in a fraction of the time. Click here to acc ess our Tm calculator. Phusion ® was developed by Finnzymes Oy now a part of Thermo Fisher Scientific
2021-6-18 · NEB()Phusion DNA Phusion DNA .cpmsbt border-bottom 1px solid #CC9966color #CC9933font-family Verdanafont-size
Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer 5x Phusion GC Buffer DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration. Quantity. 500U (2U/µL) Concentration. 2 U/µL.
While NEB develops and validates its products for various applications the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications. PHUSION® is a registered trademark and property of Thermo Fisher Scientific.
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