5 3 15bp-20bp Exnase II37℃30 1. >95 ClonExpress Entry One Step Cloning KitClonExpress One Step
2013-12-5 · ClonExpressTM II One Step Cloning Kit . . 2013/12/5 14 09 12. . vazyme. C112-01. . .
2016-8-5 · ClonExpress II One Step Cloning Kit (Vazyme Biotech Co. Ltd). Typically 20 μL of a mixture containing 4 μL of 5 CE II Buffer 2 μL of Exnase II 50 ng linear plasmid 100 ng PCR fragment and ddH2O was incubated at 37oC for 30 min and the resulting solution was used to transform chemically competent E. coli DH5α cells. Colony PCR and
2016-3-14 · recombination using the One Step Cloning Kit (Vazyme Biotech Co. Ltd). Typically 20 μL of a mixture containing 4 μL of 5 CE II Buffer 2 μL of Exnase II 50-200 ng linear plasmid 20-200 ng PCR fragment and ddH2O was incubated at 37 oC for 30
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ClonExpress UItra One Step Cloning kit (Vazyme #C115) is a novel cloning kit applicable for homologous recombination with 15 fragments. The Kit is independent of DNA ligase significantly reducing the self-ligated colonies. The enhanced Exnase and highly optimized buffer included in 2x ClonExpress Mix significantly improve the
2018-7-18 · ClonExpress® II One Step Cloning kit is a new version cloning kit based on ClonExpress® One Step Cloning technology supplied with Exnase® II and reaction buffer. Compared to the previous version Exnase® II is added a unique recombinant enhancer which can significantly improve the efficiency of recombination (100 cfu/ ng Vector) even
2021-3-15 · VazymeBiotechCo. Ltd Order global vazyme m Support global vazyme m ww w.vazyme m Forresearchuseon ly notforuseindiagnosticprocedures. M DL5 000Marker.
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1 circ-pEGFP 5 CE II Buffer 2 10 μL (Vazyme ClonExpress II One Step Cloning Kit C112-01/02) 5 CE II Buffe 2 μL circ-pEGFP vector 2 μL (50 ng) circRNA 2 μL (100 ng) Exnase® II 1 μL ddH2O 2 μL
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2020-1-16 · turer s instructions. By using of the ClonExpress II One Step Cloning Kit (Vazyme Biotech China) 0.015-pmol vectors and 0.03-pmol inserts were recombined follow-ing the manufacturer s instructions. Briefly linearized vectors inserts 5 CE II buffer and Exnase II enzyme were mixed in a final volume of 10-μl with deionized
2020-1-16 · turer s instructions. By using of the ClonExpress II One Step Cloning Kit (Vazyme Biotech China) 0.015-pmol vectors and 0.03-pmol inserts were recombined follow-ing the manufacturer s instructions. Briefly linearized vectors inserts 5 CE II buffer and Exnase II enzyme were mixed in a final volume of 10-μl with deionized
2021-6-18 · ClonExpress MultiS One Step Cloning Kit C113 ClonExpress® MultiS One Step Cloning Kit
2021-7-20 · ClonExpress II One Step Cloning Kit C112 ClonExpress® II One Step Cloning Kit
1 circ-pEGFP 5 CE II Buffer 2 10 μL (Vazyme ClonExpress II One Step Cloning Kit C112-01/02) 5 CE II Buffe 2 μL circ-pEGFP vector 2 μL (50 ng) circRNA 2 μL (100 ng) Exnase® II 1 μL ddH2O 2 μL
ClonExpress II One Step Cloning Kit-Vazyme Biotech Co. Ltd- ClonExpress® technology is a simple fast and efficient DNA seamless cloning technology which can clone the insert into any position of any vector. Linearize the vector and place the insert 5 in the forward/reverse PCR primer Introduce the end sequence of the linearized vector into the end so that the 5 and 3 ends of the PCR
5 3 15bp-20bp Exnase II37℃30 1. >95 ClonExpress Entry One Step Cloning KitClonExpress One Step
ClonExpress UItra One Step Cloning kit (Vazyme #C115) is a novel cloning kit applicable for homologous recombination with 15 fragments. The Kit is independent of DNA ligase significantly reducing the self-ligated colonies. The enhanced Exnase and highly optimized buffer included in 2x ClonExpress Mix significantly improve the
5 3 15bp-20bp Exnase II37℃30 1. >95 ClonExpress Entry One Step Cloning KitClonExpress One Step
2021-1-5 · one tube add 40 ul lipofectamine 3000 step 1 95℃ 5min step 2 95℃ 30sec step 3 56℃ 30sec step 4 72℃ 4min repeat step 2 to step 4 for 28 cycles 72℃ 10min 4℃ hold 2. we need to make them be circled. So we need to use Exnase II (C214 Vazyme) to cut the overlapped DNA segment and then recombined to circled DNA
ClonExpress II One Step Cloning Kit C112-01/02 ClonExpress®IIOneStepCloningKit 50bp-10kbDNA PCR
ClonExpress® Ultra One Step Cloning Kit is a new generation of recombinant cloning kit which can quickly and efficiently clone 1-5 fragments to any position of any vector. The highly optimized 2 ClonExpress Mix 50℃ reaction 5-15 Min completes the reorganization and the operation is
2014-4-27 · ClonExpress. Vazyme biotech co. ltd. vazyme /Web vazyme /Tel 400-600-9335 /Sales sales vazyme /Support support vazyme /Service service vazyme Vazyme Biotech Co. Ltd Version 3.2 ClonExpress TM II
72˚C for 8 min. Following enzyme digestion (Exnase™ ii Vazyme Biotech co. td. 1l µl) using loncexpress ii one Step c loning kit (Vazyme Biotech co. l td.) and sequencing the Pcr product was cloned into the i/KpnXhoi sites of the GV230 expression vector. The recombinant GV230-septin7 plasmid was confirmed by endonuclease digestion and
ClonExpress II One Step Cloning Kit C112-01/02 ClonExpress®IIOneStepCloningKit 50bp-10kbDNA PCR
کیت کلونینگ برند وازایم ؛ قیمت آنلاین، فروش و یا خرید اینترنتی کیت کلونینگ؛ قیمت آنلاین، فروش و یا خرید اینترنتی کیت کلونینگ ClonExpress MultiS One شرکت آثل طب آزمایشگاه آرکاطب روهام(ATR-MED)
2021-3-15 · VazymeBiotechCo. Ltd Order global vazyme m Support global vazyme m ww w.vazyme m Forresearchuseon ly notforuseindiagnosticprocedures. M DL5 000Marker.
Following enzyme digestion (Exnase ™ II Vazyme Biotech Co. Ltd. 1 µl) using ClonExpress II One Step Cloning kit (Vazyme Biotech Co. Ltd.) and sequencing the PCR product was cloned into the Xho I/ Kpn I sites of the GV230 expression vector. The recombinant GV230-septin7 plasmid was confirmed by endonuclease digestion and DNA sequencing
2020-12-21 · ClonExpress II One Step Cloning Kit (Vazyme Biotech . no. C112-02) (the 18.000-bp product purified in Step 5) with the ClonExpress II One Step Cloning Kit using the following reaction mix
کیت کلونینگ برند وازایم ؛ قیمت آنلاین، فروش و یا خرید اینترنتی کیت کلونینگ؛ قیمت آنلاین، فروش و یا خرید اینترنتی کیت کلونینگ ClonExpress MultiS One شرکت آثل طب آزمایشگاه آرکاطب روهام(ATR-MED)
ClonExpress® Ultra One Step Cloning Kit is a new generation of recombinant cloning kit which can quickly and efficiently clone 1-5 fragments to any position of any vector. The highly optimized 2 ClonExpress Mix 50℃ reaction 5-15 Min completes the reorganization and the operation is
کیت کلونینگ برند وازایم ؛ قیمت آنلاین، فروش و یا خرید اینترنتی کیت کلونینگ؛ قیمت آنلاین، فروش و یا خرید اینترنتی کیت کلونینگ ClonExpress MultiS One شرکت آثل طب آزمایشگاه آرکاطب روهام(ATR-MED)
2020-6-29 · Homologous recombination (lonExpress II One Step loning Kit Vazyme orp. at# 112) 1. Calculate DNA amount homologous recombination needed according to the formula. In order to ensure the accuracy we recommend to dilute the linear vector
2019-3-21 · Ex nase II at37℃for 30 min(F ig.1.). The cloningpro duc tsc anthen be directlytran sfo rmed to com petent cellswith ap ositive rate >95 . ClonExpress MultiS One Step Cloning Kit is anew version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS
2018-7-18 · ClonExpress® II One Step Cloning kit is a new version cloning kit based on ClonExpress® One Step Cloning technology supplied with Exnase® II and reaction buffer. Compared to the previous version Exnase® II is added a unique recombinant enhancer which can significantly improve the efficiency of recombination (100 cfu/ ng Vector) even
2021-1-5 · one tube add 40 ul lipofectamine 3000 step 1 95℃ 5min step 2 95℃ 30sec step 3 56℃ 30sec step 4 72℃ 4min repeat step 2 to step 4 for 28 cycles 72℃ 10min 4℃ hold 2. we need to make them be circled. So we need to use Exnase II (C214 Vazyme) to cut the overlapped DNA segment and then recombined to circled DNA
One method is two-step PCR extending megaprimer along with vector to amplify plasmid DNAs ligating the DNAs in blunt-end by T4 DNA ligase and transforming the ligaed DNAs into host cells (Figure 1 middle). Although efficient for creating over 10 transformants/ng the method is compromised by constructing occasionally scar plasmids i.e
ClonExpress II One Step Cloning Kit-——-ClonExpress®IIOneStepCloningKit 50bp-10kbDNA
> > ClonExpress® II One Step Cloning Kit ClonExpress® II One Step Cloning Kit. Drucken Melden Sie sich an Menge Katalog-Nummer C112-01. Size 25rxn. Marke Vazyme. ClonExpress® technology is a simple fast and efficient DNA seamless cloning technology which can clone the insert into any position of any vector. The highly optimized
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